Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1’in Real Time-PCR ile Expresyon Profilinin Çıkarılması
| Autor: | Mehmet Ulas Cinar, Aytül Kürüm, Turgay Deprem, Hakan Kocamiş |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty GPX1 Ethanol General Veterinary business.industry Sterile water Streptozotocin chemistry.chemical_compound Enzyme Endocrinology Real-time polymerase chain reaction chemistry Capsaicin Internal medicine Gene expression medicine business medicine.drug |
| Zdroj: | Kafkas Universitesi Veteriner Fakultesi Dergisi. |
| ISSN: | 1300-6045 |
| DOI: | 10.9775/kvfd.2014.12233 |
| Popis: | The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P |
| Databáze: | OpenAIRE |
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