The Effect of Some Natural Cytotoxic Peptides on Tumor Cells

Autor:	Mihaiela Loredana Dragos, Magda Badescu, Bogdan Mihail Diaconescu, Teodor Stefanache, Manuela Ciocoiu, Daniela Jitaru, Madalina Mocanu, Codruta Badescu
Rok vydání:	2018
Předmět:	Chemistry Materials Science (miscellaneous) Process Chemistry and Technology Materials Chemistry General Engineering Cancer research Cytotoxic T cell Tumor cells General Chemistry General Medicine General Pharmacology Toxicology and Pharmaceutics General Biochemistry Genetics and Molecular Biology
Zdroj:	Revista de Chimie. 69:597-601
ISSN:	2668-8212 0034-7752
Popis:	Many studies have highlighted the anti-tumor properties of some natural peptides known to have antimicrobial virtues. In this study, we evaluated the tumoricidal potential of dermaseptin, defensin, cecropin A and B on tumor cell lines: M14K (human mesothelioma). The viability study was performed using PE V Annexin and 7-AAD (7-amino-actinomycin D) (BD Pharmingen). This test was used to detect and measure apoptosis by flow cytometry technique. The experimental results of our study revealed that the cytotoxic effects of the four peptides depend on their concentration. In this in vitro experimental study, we found that the cytotoxic effect of the four cytotoxic peptides used depended on their concentration in the tumor cell culture medium, being significant at concentrations of 120mM and maintained at concentrations of 60�M. At 30�M concentrations these tumoricidal effects were insignificant. Of all the studied peptides, dermaseptin has the most powerful effect and the weakest effect b - defensin - 1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2ed5bc7c330a5e4b57f61137d5b21c19 https://doi.org/10.37358/rc.18.3.6157 Zobrazit plný text záznamu