Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer byCupriavidus eutrophusB10646
| Autor: | Tatiana G. Volova, Aleksey G. Sukovatiy, Natalia O. Zhila, Daria A. Syrvacheva |
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| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Molar General Chemical Engineering chemistry.chemical_element 02 engineering and technology Mole fraction 01 natural sciences Inorganic Chemistry chemistry.chemical_compound 010608 biotechnology Copolymer Organic chemistry Waste Management and Disposal chemistry.chemical_classification Strain (chemistry) biology Renewable Energy Sustainability and the Environment Chemistry Organic Chemistry Polymer 021001 nanoscience & nanotechnology biology.organism_classification Pollution Nitrogen Fuel Technology Monomer Cupriavidus 0210 nano-technology Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 91:416-425 |
| ISSN: | 0268-2575 |
| Popis: | BACKGROUND P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5–6 g L-1) and high content of the polymer (60–75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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