Abstract 061: Cell Fate Changes During Tubular Damage and Regeneration in the Mouse Kidney
| Autor: | R. A. Gomez, Maria Luisa S. Sequeira-Lopez, Vidya K. Nagalakshmi, Minghong Li |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Kidney
Pathology medicine.medical_specialty urogenital system business.industry Regeneration (biology) Chronic renal disease Degeneration (medical) Cell fate determination Interstitial fibrosis urologic and male genital diseases medicine.disease medicine.anatomical_structure Fibrosis Internal Medicine Mouse Kidney Medicine business |
| Zdroj: | Hypertension. 68 |
| ISSN: | 1524-4563 0194-911X |
| Popis: | Tubular degeneration, loss of renal tubules and interstitial fibrosis due to kidney injury lead to chronic renal disease and hypertension. Using a partial unilateral ureteral obstruction (pUUO) model in neonatal mice, we analyzed the fate cell changes that occur during obstruction and during recovery following the release of UUO. We traced the fate of cells derived from the renal stroma, cap mesenchyme and ureteric bud epithelium using Foxd1-Cre, Six2-Cre and HoxB7-Cre mice respectively, crossed with double fluorescent reporter (mT/mG) mice. pUUO was performed 24-36h after birth (n=84). In a group of pups (n=37), the obstruction was released after seven days. Sham operated animals (n=35) were used as controls. Lineage tracing revealed that Foxd1-derived interstitial pericytes acquired α-smooth muscle actin expression and underwent significant expansion due to pUUO (fibrotic area 91.06+/-6.77 %). Release of obstruction resulted in complete resolution of fibrotic areas (0.00%; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|