Development and application of ELISA assays for the detection of two members of the familyLuteoviridaeinfecting legumes:Pea enation mosaic virus(genusEnamovirus) andBean leafroll virus(genusLuteovirus)

Autor:	B. Vemulapati, Sanford D. Eigenbrode, Hanu R. Pappu, K. L. Druffel, D. Husebye
Rok vydání:	2014
Předmět:	Antiserum food.ingredient Serial dilution biology viruses Luteovirus food and beverages Pea enation mosaic virus biology.organism_classification Virology Virus Enamovirus Serology food Agronomy and Crop Science Gene
Zdroj:	Annals of Applied Biology. 165:130-136
ISSN:	0003-4746
Popis:	An antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) method was developed and validated for the detection of Bean leafroll virus (BLRV) and Pea enation mosaic virus (PEMV), two of the important viral pathogens of several legume crops. The coat protein (CP) gene of each of the viruses was bacterially expressed as a fusion protein containing an N-terminal hexa-histidine tag and used as an antigen to produce antisera in rabbits. The antiserum to BLRV could detect the virus in leaf samples in up to 1:1000 dilution, and the PEMV antiserum detected the homologous virus in leaf samples of dilutions up to 1:6400. No serological cross-reactivity was observed between anti-BLRV and anti-PEMV sera. The ACP-ELISA assays were then used for estimating the prevalence of these two viruses in alfalfa, pea and vetch over a three-state area in the US Pacific Northwest over a 2-year period and virus incidence was mapped. Availability of rapid and sensitive ELISA assays facilitate virus disease mapping efforts and screening germplasm for virus resistance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2ea0580e5fcf5bfb6eaeb09e2ea5e5f3 https://doi.org/10.1111/aab.12126 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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