Simultaneous single-step immobilization of Candida antarctica lipase B and incorporation of magnetic nanoparticles on poly(urea-urethane) nanoparticles by interfacial miniemulsion polymerization
| Autor: | Débora de Oliveira, Viviane Chiaradia, Pedro Henrique Hermes de Araújo, Alexsandra Valério, Claudia Sayer |
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| Rok vydání: | 2016 |
| Předmět: |
Immobilized enzyme
Nanoparticle Bioengineering 02 engineering and technology 01 natural sciences Biochemistry Catalysis chemistry.chemical_compound Polymer chemistry Ethyl oleate chemistry.chemical_classification biology 010405 organic chemistry Process Chemistry and Technology equipment and supplies 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Miniemulsion Polymerization chemistry Propionate Magnetic nanoparticles Candida antarctica 0210 nano-technology Nuclear chemistry |
| Zdroj: | Journal of Molecular Catalysis B: Enzymatic. 131:31-35 |
| ISSN: | 1381-1177 |
| DOI: | 10.1016/j.molcatb.2016.06.004 |
| Popis: | In this work Candida antarctica lipase B (E.C: 3.1.1.3), was immobilized on magnetic poly(urea-urethane) nanoparticles (MNPs-PUU) in a single-step during the interfacial miniemulsion polymerization. Transmission electron microscopy images showed the morphology of synthesized magnetic nanoparticles encapsulated in poly(urea-urethane) nanoparticles and fluorescence microscopy images confirmed the enzyme immobilization onto MNPs-PUU. After the immobilization process, the immobilized enzyme on support (MNPs-PUU) was attracted by an external magnetic field and used as biocatalyst for the synthesis of esters ethyl oleate, geranyl propionate and geranyl oleate. Ester conversions above 85% were obtained for all systems based on the free fatty acids contents measured by titration until pH 11. |
| Databáze: | OpenAIRE |
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