Preparation of immuno-probes based on europium-chelate-adsorbed silica nanoparticles and magnetic nanoparticles and their application in detection of hepatitis B surface antigen

Autor:	Rong Xu, Ziyang Hu, Lian Xie, Zai-Zhen Wu, Xing-Ru Dou, Hong-Ping Xie, Xu-Ting Zhu
Rok vydání:	2012
Předmět:	General Chemical Engineering Sonication Layer by layer General Engineering Analytical chemistry Cationic polymerization chemistry.chemical_element Polyelectrolyte Analytical Chemistry chemistry.chemical_compound Adsorption chemistry Magnetic nanoparticles Europium Acrylic acid Nuclear chemistry
Zdroj:	Analytical Methods. 4:3810
ISSN:	1759-9679 1759-9660
DOI:	10.1039/c2ay25686j
Popis:	In this paper, a simple and reproducible method to synthesize intense fluorescence and photostable silica nanoparticles has been developed. Firstly, silica nanoparticles were prepared based on the Stober method, and hydrophobic europium chelates were adsorbed into their micro-holes by ultrasonication and phase-transfer solvent. Then they were modified by cationic polyelectrolyte polydiallyldimethylammonium chloride (PDAC) and anionic polyelectrolyte poly(acrylic acid) (PAA) layer by layer. Using them as detection probes and magnetic nanoparticles as capture probes, a novel immunoassay for determination of hepatitis B surface antigen (HBsAg) was proposed. Under the optimal experimental conditions, the immuno-probes performed with a good linear range over 10–200 ng mL−1 with a calibration curve equation of y = −549.24 + 652.44x (R = 0.9901). Based on the hydrophobicity and intense fluorescence europium chelates absorbed into silica nanoparticles, one-step fluorescence immunoassay has been realized and may have potential applications in clinical assays.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e73dd6d4026d62637badb7da3cbb411 https://doi.org/10.1039/c2ay25686j Zobrazit plný text záznamu