Gel filtration chromatography analysis and modeling the process of pullulan depolymerization

Autor:	Stanko Zerajic, Ivana M. Savic, Goran Nikolić, Ivan M. Savic
Rok vydání:	2012
Předmět:	chemistry.chemical_compound Chromatography Materials science Polymers and Plastics chemistry Depolymerization General Chemical Engineering Scientific method Size-exclusion chromatography Materials Chemistry Pullulan
Zdroj:	Journal of Polymer Engineering. 32:225-233
ISSN:	2191-0340 0334-6447
DOI:	10.1515/polyeng-2011-0104
Popis:	Different fractions of pullulan oligosaccharide can be applied in the synthesis of pharmaceutical active compounds. Thus, the object of this study was to model the depolymerization process of pullulan polysaccharide with the change of pH, temperature and depolymerization time. The samples were analyzed by gel filtration chromatography (GFC). The chromatography separation of polymer fractions was achieved by using a Zorbax PSM-300 HP-SEC column (250×6.2 mm, 5 μm) at a temperature of 25°C. The flow rate of the mobile phase (redistilled water) was 1 cm3 min-1. The fractions were recorded by a refractive index detector. The obtained data were converted by thin plate spline interpolation function into matrixes. By the mathematical processing matrixes, the models of molar mass distribution in the function of pH, temperature and depolymerization time were obtained. The intervals of depolymerization parameters can be defined for obtaining the desirable pullulan oligomers, which have the efficient applications in macromolecule technology.
Databáze:	OpenAIRE
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