Application of gold nanoparticles as contrast agents in confocal laser scanning microscopy
| Autor: | Sergey A. Piletsky, I. S. Kozhevnikov, Igor Victorovich Meglinski, G. G. Akchurin, B A Veksler, A. Lemelle |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Materials science
genetic structures Physics and Astronomy (miscellaneous) Image quality media_common.quotation_subject fungi Nanoparticle Nanotechnology Fluorescence law.invention Autofluorescence Colloidal gold Confocal microscopy law Contrast (vision) sense organs Instrumentation Image resolution Biomedical engineering media_common |
| Zdroj: | Laser Physics Letters. 6:71-75 |
| ISSN: | 1612-202X 1612-2011 |
| DOI: | 10.1002/lapl.200810091 |
| Popis: | Confocal laser scanning microscopy (CLSM) is a modern high-resolution optical technique providing detailed image of tissue structure with high (down to microns) spatial resolution. Aiming at a concurrent improvement of imaging depth and image quality the CLSM requires the use of contrast agents. Commonly employed fluorescent contrast agents, such as fluorescent dyes and proteins, suffer from toxicity, photo-bleaching and overlapping with the tissues autofluorescence. Gold nanoparticles are potentially highly attractive to be applied as a contrast agent since they are not subject to photo-bleaching and can target biochemical cells markers associated with the specific diseases. In current report we consider the applicability of gold nano-spheres as a contrast agent to enhance quality of CLSM images of skin tissues in vitro versus the application of optical clearing agent, such as glycerol. The enhancement of CLSM image contrast was observed with an application of gold nano-spheres diffused within the skin tissues. We show that optical clearing agents such as a glycerol provide better CLSM image contrast than gold nano-spheres. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text