Bifactorial Design Applied to Recombinant Protein Expression

Autor:	V. Martinez-Luaces, G. Guineo-Cobs, H. Massaldi, B. Velazquez, A. Chabalgoity
Rok vydání:	2021
Předmět:	Strain (chemistry) Chemistry Laboratory scale medicine.disease_cause Bioinformatics Protein expression law.invention Biochemistry law Yield (chemistry) Streptococcus pyogenes medicine Recombinant DNA Protein biosynthesis Escherichia coli
Zdroj:	Journal of Data Science. 4:247-255
ISSN:	1683-8602 1680-743X
Popis:	We have studied the effect of several factors that influence recombinant protein production, by using the expression of recombinant streptolysin-O as our model. This protein, produced by Streptococcus pyogenes, is important in the biotechnological industry, where it is used to produce immunodiagnostic reagents. In order to improve the yield of this protein, we tried an alternative production method using strains of Escherichia coli and recombinant DNA technology. We have evaluated this method at the laboratory scale, taking into account factors such as inductor concentration, temperature of induction, proportion of culture medium volume to total flask volume, and strain of Escherichia coli used. To this end we applied techniques of experimental design, particularly a ”fixed-effects bifactorial design”, with the expression level of recombinant streptolysin-O in E. coli being the response to the factors. All the effects studied were found to be significant and relevant to the economics of the protein production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e50d21d3ec8d78d9ff38b86cd0cc082 https://doi.org/10.6339/jds.2006.04(2).240 Zobrazit plný text záznamu