Split Dapoxyl Aptamer for Sequence-Selective Analysis of Nucleic Acid Sequence Based Amplification Amplicons
| Autor: | Adam J. Reed, Yulia V. Gerasimova, Nanami Kikuchi, Dmitry M. Kolpashchikov |
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| Rok vydání: | 2019 |
| Předmět: |
Hybridization probe
Aptamer 010401 analytical chemistry Loop-mediated isothermal amplification Nucleic acid amplification technique Amplicon 010402 general chemistry 01 natural sciences Molecular biology 0104 chemical sciences Analytical Chemistry Nucleic acid thermodynamics chemistry.chemical_compound chemistry TaqMan DNA |
| Zdroj: | Analytical Chemistry. 91:2667-2671 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b03964 |
| Popis: | Hybridization probes have been used for the detection of single nucleotide variations (SNV) in DNA and RNA sequences in the mix-and-read formats. Among the most conventional are Taqman probes, which require expensive quantitative polymerase chain reaction (qPCR) instruments with melting capabilities. More affordable isothermal amplification format requires hybridization probes that can selectively detect SNVs isothermally. Here we designed a split DNA aptamer (SDA) hybridization probe based on a recently reported DNA sequence that binds a dapoxyl dye and increases its fluorescence (Kato, T.; Shimada, I.; Kimura, R.; Hyuga, M., Light-up fluorophore-DNA aptamer pair for label-free turn-on aptamer sensors. Chem. Commun. 2016, 52, 4041−4044). SDA uses two DNA strands that have low affinity to the dapoxyl dye unless hybridized to abutting positions at a specific analyte and form a dye-binding site, which is accompanied by up to a 120-fold increase in fluorescence. SDA differentiates SNV in the inhA gene of Myc... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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