| Autor: |
Yan Zhou, Zhenwei Deng, Ting Pan, Guohao Chen, Yongchang Cai, Yuxin Tang, Yijun Wang, Yucheng Wang, Ruiping Li, Libo Li |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1342705/v1 |
| Popis: |
Background: Long noncoding RNAs (lncRNAs) are a class of noncoding RNAs implicated in the development of many malignancies, including gastric cancer (GC). Herein, we investigated the functions and molecular mechanisms of non-coding RNA activated by DNA damage (NORAD) in GC. Methods: NORAD expression at the mRNA levels was determined by qRT-PCR assay. Cell proliferation, migration and invasion was detected by CCK-8 assay, in vivo tumor formation assay and Transwell assay. NORAD location was detected by nucleocytoplasmic fractionation assay. The interaction between NORAD and miR-204-5p/KMT2D axis was verified by dual-luciferase reporter gene assay and RIP assay. Results: NORAD was upregulated in GC tissues and cell lines. NORAD silencing repressed cell proliferation and G2/M cell cycle transition in GC. NORAD could act as a ceRNA of miR-204-5p and regulate KMT2D expression. Conclusions: Our study demonstrated that NORAD played as an oncogene in GC, which might provide a new therapeutic target for the disease. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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