Genetic characterization ofSalix albaL. andSalix fragilisL. by means of different PCR-derived marker systems

Autor: Paolo Paiero, Stefano Meneghetti, Margherita Lucchin, Gianni Barcaccia
Rok vydání: 2007
Předmět:
Zdroj: Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 141:283-291
ISSN: 1724-5575
1126-3504
Popis: Salix alba L. and Salix fragilis L. are two closely related willow species whose phenotypic features, showing a large and continuous variation, have a low diagnostic value for identifying pure species and interspecific hybrids. In this paper, the effectiveness of different multilocus PCR-based molecular markers, such as I-SSRs, RAPDs and AFLPs in detecting genetic polymorphisms able to discriminate the two willow species was evaluated by analysing a set of 12 reference samples. Three genetic similarity indexes, Dice, Jaccard and Simple Matching coefficient, were used for all possible pairwise comparisons of individuals, revealing the same trend of variation within and between species when different marker systems were used. Cluster analysis, based on Dice genetic similarity coefficient, clustered the individuals of S. alba and S. fragilis into two distinct subgroups, indicating that the gene pools are well differentiated. Moreover, a number of private alleles for each marker system allowed the di...
Databáze: OpenAIRE
Nepřihlášeným uživatelům se plný text nezobrazuje