A method for screening potential antioxidant activity
| Autor: | Kazuyoshi Masaki, Isao Karube, Zvy Dubinsky, Toshikazu Nishida, Ritsuko Matsukawa |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Chromatography Antioxidant Immobilized enzyme biology medicine.medical_treatment Substrate (chemistry) chemistry.chemical_element Bioengineering General Medicine Applied Microbiology and Biotechnology Oxygen law.invention Lipoxygenase chemistry.chemical_compound Enzyme chemistry law biology.protein medicine Agarose Clark electrode Biotechnology |
| Zdroj: | Journal of Biotechnology. 51:149-155 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/0168-1656(96)01611-2 |
| Popis: | A flow injection system for screening antioxidant activity was developed using lipoxygenase enzyme immobilized on 1,1′-carbonydiimidazole activated cross-linked beaded agarose support. Oxygen consumption and hydroperoxide formation were measured using a Clark oxygen electrode and a UV detector located downstream relative to the flow column. The measurement of the rate of oxygen consumption and hydroperoxide formation provide a unique dual signal method to detect antioxidant activity. Antioxidant detection is demonstrated for microalgal extracts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect