Le thiocyanate serait un inhibiteur puissant de l'oxalate oxydase dans le dosage de l'oxalate urinaire

Autor:	S Lartillot, M.A.Saka Amini, J. J. Vallon
Rok vydání:	1991
Předmět:	Chromatography Thiocyanate Immobilized enzyme Chemistry Oxalate oxidase Metabolite Cyanide Ion chromatography Enzyme electrode Biochemistry Oxalate Analytical Chemistry chemistry.chemical_compound Environmental Chemistry Spectroscopy
Zdroj:	Analytica Chimica Acta. 245:129-132
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(00)80211-1
Popis:	( Urinary thiocyanate appears to be a powerful inhibitor of oxalate oxidase. ) The application of an oxalate oxidase electrode to urinary oxalate determination allowed us to observe, after repeated use, an irreversible inhibition of the immobilized enzyme. All known urinary inhibitors previously described do not show such an irreversible phenomenon. Attempts to separate oxalate from the inhibitor by a Sephadex G-10 column were unsuccessful. The inhibitor could be the thiocyanate ion, which is always present in urine as a metabolite of cyanide. Thiocyanate isolation from oxalate is possible by using an anionic-exchange column.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e3b3b6e85ce2f11ad748bc6f5a21aa7 https://doi.org/10.1016/s0003-2670(00)80211-1 Zobrazit plný text záznamu