| Autor: |
Bau-Lian Huang, Chi-Jen Lin, Toshio Murashige, Li-Chun Huang, Ching-I Kuo |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Scientia Horticulturae. 91:111-121 |
| ISSN: |
0304-4238 |
| DOI: |
10.1016/s0304-4238(01)00240-0 |
| Popis: |
A mericloning protocol that enables shoot multiplication and rooting of Paphiopedilum orchids in one step or in the same medium was developed by systematically assessing and modifying the nutrient supplements employed earlier for shoot proliferation by Huang [Am. Orchid Soc. Bull. 57 (1988) 274]. Shoots of aseptically established seedlings obtained from a commercial breeder were used as explants. The new combination of supplements to the MS [Phyiol. Plant. 15 (1962) 473] based medium consists of MT [Proc. First Intl. Citrus Symp. 3 (1969) 1155] vitamins, glycine and inositol; 13 μM BA (N6-benzyladenine); 1.6 μM NAA (α-naphthaleneacetic acid); 0.15 mM adenine sulfate·2H2O; 1.23 mM NaH2PO4·H2O; 0.18 M sucrose; and 15% (v/v) coconut milk from very young fruits. The coconut milk can be substituted with 1 g l−1 casein hydrolysate or 10 g l−1 potato tuber sections. The investigation also disclosed that TDZ (N-phenyl-N′-1,2,3-thiadiazol-5-yl urea) inhibited shoot proliferation and rooting, and maltose depressed rooting. The protocol enables doubling the number of Paphiopedilum plants every 12 weeks. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect