Potent Inhibition of the Proinflammatory Responses of Human Polymorphonuclear Cells by Prostaglandin E1 but not by PGI2 or Carbacyclin
| Autor: | Toshiaki Akira, Kazuki Murakami, Teruaki Imada, Norifumi Nakamura, Ruriko Ikegawa |
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| Rok vydání: | 1998 |
| Předmět: |
Pharmacology
IBMX Physiology medicine.medical_treatment Prostaglandin Chemotaxis Prostacyclin Cell Biology respiratory system Biology Biochemistry Umbilical vein Proinflammatory cytokine chemistry.chemical_compound chemistry cardiovascular system medicine lipids (amino acids peptides and proteins) Prostaglandin E1 circulatory and respiratory physiology medicine.drug Prostaglandin E |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 55:95-107 |
| ISSN: | 1098-8823 |
| DOI: | 10.1016/s0090-6980(98)00011-2 |
| Popis: | The effects of prostaglandin E1 (PGE1) on adhesion of activated human polymorphonuclear cells (HPMNs) to human umbilical vein endothelial cells (HUVECs) and on chemotaxis of HPMNs were compared with those of prostaglandin I2 (PGI2) or its chemically stable analog carbacyclin. Although HUVECs treated with PGI2 inhibited only fMLP-activated HPMN adhesion, both HUVECs and HPMNs treated with PGE1 equally inhibited HPMN adhesion, thus indicating the appropriate reactions to endothelium-leukocyte interaction by PGE1. In the presence of a nonspecific phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), PGE1 inhibited HPMN chemotaxis induced by either fMLP or LTB4, whereas both PGI2 and carbacyclin had no effect. To examine whether the response of PMNs to PGs is species-specific, intracellular cAMP accumulation in response to PGs was determined in human, guinea-pig, and rat isolated PMNs. Although PGE1 increased cAMP levels in all PMNs tested, both PGI2 and carbacyclin increased cAMP only in rat PMNs. These results demonstrate that HPMN cAMP formation can be affected by PGE1 but not by PGI2. The findings help our understanding of the clinical use of PGs in patients with peripheral arterial occlusive diseases. |
| Databáze: | OpenAIRE |
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