Modeling and Model Based Feeding Control for Pichia pastoris Fed-Batch Cultivation

Autor:	Jingqi Yuan, B.K. He, H.T. Ren, L.M. Ren, J.H. Deng
Rok vydání:	2004
Předmět:	biology Biomass biology.organism_classification Pichia pastoris chemistry.chemical_compound chemistry Biochemistry Glycerol Standard protocol Bioreactor Food science Methanol Constant (mathematics) Protein concentration
Zdroj:	IFAC Proceedings Volumes. 37:451-456
ISSN:	1474-6670
DOI:	10.1016/s1474-6670(17)38773-6
Popis:	A structured kinetic model, which takes account of major metabolic pathways of glycerol and methanol in Pichia pastoris, is presented. Based on the combined kinetic and bioreactor model, feeding profiles of methanol are detennined with the aim of maintaining constant specific growth rate during production stage. Compared with the decreasing type of specific growth rate resulted from constant feeding profile in the standard protocol, the constant specific growth rate is believed to be advantageous for improving the productivity. Experimental results indicate that simulations of biomass and protein concentration agree well with the measured data, and the specific growth rates were successfully controlled at various set points.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e278a55242b68f0c8224c0586348a03 https://doi.org/10.1016/s1474-6670(17)38773-6 Zobrazit plný text záznamu