Collapse of Neuronal Energy Balance As a Basis of L-Homocysteine Neurotoxicity
| Autor: | T. V. Karelina, P. A. Abushik, L. S. Sitnikova, Sergei M. Antonov, Yu. D. Stepanenko, Rashid Giniatullin, D. A. Sibarov, M. A. Ivanova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Membrane potential medicine.medical_specialty Cerebellum Chemistry Biophysics Glutamate receptor Neurotoxicity Cell Biology Mitochondrion medicine.disease Biochemistry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology medicine.anatomical_structure nervous system Apoptosis Internal medicine medicine NMDA receptor 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 12:360-368 |
| ISSN: | 1990-7494 1990-7478 |
| Popis: | Using fluorescence detection methods, neurotoxic effects of L-homocysteine (HCY), L-glutamate (Glu), and N-methyl-D-aspartate (NMDA) on primary culture of rat cerebellar neurons were compared and the agonist-evoked intracellular Ca2+ responses and changes in mitochondrial membrane potential were studied. Long-term (5 h) action of HCY, Glu, or NMDA caused neuronal apoptosis and necrosis that was followed by a decrease of quantity of live cells to 40%. It was revealed using Fluo-3 that neurons differed by intracellular Ca2+ responses to 2-min applications of HCY. In response to all studied agonists, a brief peak or gradual increase of intracellular Ca2+ concentration was observed. Some neurons did not respond to HCY, but all responded to Glu and NMDA. A prolonged (60 min) treatment with agonists caused a rapid or delayed Ca2+ overload, while only a small portion of neurons were able to compensate the intracellular Ca2+ elevation. Six-minute applications of HCY or Glu to neurons induced similar changes of mitochondrial potential (φmit) measured by rhodamine123. In this protocol, the ability of the NMDA receptor agonists to cause the mitochondrial dysfunction could be arranged in the following order: NMDA > Glu = HCY. After a 60-min treatment the observed difference vanished because all of the agonists reduced φmit so that an uncoupling agent FCCP did not cause any additional changes in φmit. Thus, HCY-induced neurotoxicity in cerebellar neurons is comparable to that of Glu. In this feature cerebellar neurons differ from cortical neurons, in which HCY did not significantly change φmit during short-term application. This difference could be related with peculiarities of the HCY action on NMDA receptor subtypes expressed by cerebellar neurons. |
| Databáze: | OpenAIRE |
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