Development and Evaluation of Robust RP-HPLC Method for Gliclazide Estimation Integrating Box Behnken Design

Autor:	Mansi Dholakia, Hardik B. Rana, Vaishali Thakkar, Mukesh C. Gohel, Saloni Desai, Tejal R. Gandhi, Kalpana G. Patel
Rok vydání:	2019
Předmět:	Isocratic elution Materials science Chromatography Uv detector Box–Behnken design Ich guideline Dosage form Volumetric flow rate medicine Pharmacology (medical) Gliclazide Pharmacology Toxicology and Pharmaceutics (miscellaneous) Retention time medicine.drug
Zdroj:	Research Journal of Pharmacy and Technology. 12:135
ISSN:	0974-360X 0974-3618
Popis:	A selective, precise and accurate RP-HPLC method was developed and validated for rapidly determining the quantity of gliclazide in pharmaceutical dosage form. Isocratic elution was employed on a PhenomenexC18 column at a flow rate of 1.2 ml/min. The mobile phase consisted of methanol and 0.02 M potassium dihydrogen orthophosphate (70:30 %v/v). Gliclazide was detected at 210 nm wavelength using UV detector. Linearity was observed in concentration range of 1-100 µg/ml. Retention time of gliclazide retention time was found to be 6.06 min. Validation of developed method was performed as per the ICH guideline. Experimental design approach was used for confirmation of robustness by using Box-Behnken Design with a total 17 runs. The newer method is found to be promising for the estimation of gliclazide in unit or bulk pharmaceutical dosage form.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e219aee1a2322a37d1c0086ea23a536 https://doi.org/10.5958/0974-360x.2019.00026.x Zobrazit plný text záznamu