System peaks and optimization of anion separation in capillary electrophoresis with non-reversed electroosmotic flow

Autor:	V. V. Sursyakova, Anatoliy I. Rubaylo, Galina V. Burmakina, S. N. Kalyakin
Rok vydání:	2012
Předmět:	Analyte Chromatography Chemistry Capillary action Analytical chemistry Electrolyte Cathode Analytical Chemistry Ion law.invention Volumetric flow rate Electropherogram Capillary electrophoresis law
Zdroj:	Journal of Analytical Chemistry. 67:783-789
ISSN:	1608-3199 1061-9348
Popis:	We studied system peaks present in the electropherograms obtained in the separation of anions by capillary electrophoresis with indirect spectrophotometric detection and cathode electroosmotic flow (EOF) with a chromate background electrolyte. The system peaks correspond to the zones with changed concentration of the background electrolyte; they formed when the zones of each analyte passed through the outlet of the capillary and then moved towards the EOF detector. It has been revealed that the height and area of the system peaks linearly depends on the concentration of the corresponding anion and the areas of the system peaks can achieve 10% of the anion peak area. An algorithm has been proposed for the determination of the optimal conditions for anion separation using hydrodynamic pressure for the regulation of the EOF flow rate. This algorithm prevents the overlapping of the anion and system peaks.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2e1a520cb4bce19f621656262112b158 https://doi.org/10.1134/s1061934812090080 Zobrazit plný text záznamu Plný text ve formátu PDF