Synchronous sensing of three conserved sequences of Zika virus using a DNAs@MOF hybrid: experimental and molecular simulation studies
| Autor: | Bao-Ping Xie, Jin-Xiang Chen, Bin Sun, Gui-Hua Qiu, Zi-Feng Yang, Wen-Hua Zhang, Zhi-Hong Jiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
biology RNA 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences DNA sequencing 0104 chemical sciences Conserved sequence Zika virus Inorganic Chemistry chemistry.chemical_compound Crystallography chemistry Pyridinium 0210 nano-technology Selectivity Hybrid material |
| Zdroj: | Inorganic Chemistry Frontiers. 6:148-152 |
| ISSN: | 2052-1553 |
| DOI: | 10.1039/c8qi01031e |
| Popis: | A metal–organic framework of Cu(II) supported by a zwitterionic pyridinium dicarboxylate and 4,4′-bipyridine mixed ligands was prepared and impregnated with three dye-labeled DNA sequences with fixed wavelength differences. This hybrid material formed is capable of the synchronous detection of three conserved Zika virus RNA sequences with high sensitivity and selectivity, and low detection limits of 0.56 ± 0.02, 0.16 ± 0.04, and 0.19 ± 0.05 nM, without any cross-reactions, as confirmed by both experimental and molecular simulation studies. |
| Databáze: | OpenAIRE |
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