Selective RP-HPLC DAD method for determination of tenofovir fumarate and emtricitabine in bulk powder and in tablets

Autor:	Azza A. Gazy, Heba K. Ashour, Rasha A. Shaalan, Mohammad H. Abdelhay
Rok vydání:	2015
Předmět:	Peak area Analyte Chromatography Tenofovir Rp hplc dad General Chemistry Emtricitabine Diode array High-performance liquid chromatography chemistry.chemical_compound chemistry medicine Triethylamine medicine.drug
Zdroj:	Acta Chromatographica. 27:41-54
ISSN:	2083-5736 1233-2356
DOI:	10.1556/achrom.27.2015.1.4
Popis:	A simple and selective liquid chromatographic method was developed for the simultaneous determination of tenofovir disoproxil fumarate (TEN) and emtricitabine (EMT) in combined tablets. The method is based on separation of TEN and EMT on a Zorbax SB-C8 column, 5 μm, 4.6 × 250 mm, with a mobile phase consisting of 50 mM disodium hydrogen phosphate-acetonitrile (50:50, v/v). The mobile phase contains 0.1% triethylamine (TEA) and was adjusted to pH 6.0. Quantification of the analytes is achieved with diode array — ultraviolet detector (DAD-UV) at 260 and 280 nm for TEN and EMT, respectively, based on peak area. Different variables affecting the method were carefully investigated and optimized. Reliability and analytical performance of the proposed method, including linearity, range, precision, accuracy, and detection and quantitation limits, were statistically validated. The high-performance liquid chromatography (HPLC) method was successfully applied for determination of each drug in their binary tablets.
Databáze:	OpenAIRE
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