Immunotherapy of experimental autoimmune encephalomyelitis by adoptive transfer of B cells stimulated with MOG (50.4)
| Autor: | Jeong-Su Do, Eun-Jung Song, Hyo-Jung Seo, Jin-Ki Hwang, Jun-Hee Kim, Sung-Hye Shin, Pyung Han Hwang, Ho Keun Yi, Sang-Yun Nam |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:50.4-50.4 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.182.supp.50.4 |
| Popis: | The efficacy of adoptive transfer of activated B cells in an experimental autoimmune encephalomyelitis (EAE) model was investigated. B cells were prepared from C57BL/6 mice immunized with myelin oligodendrocyte glycoprotein (MOG 323-339) in CFA. Primary (1o) or secondary (2 o) stimulation of purified B cells was performed with MOG, LPS, and anti-CD40. EAE was induced by immunization with MOG plus M. tuberculosis and pertussis toxin. Adoptive transfer of 2 o B cells on day-1(D-1) of MOG immunization significantly prevented EAE development in a dose-dependent manner, but not 1o B cells. Adoptive transfer on D13 of immunization also suppressed EAE progress. At day 50, controls and treated mice were reimmunized with MOG and the results showed that adoptive transfer of activated 2o B cells induced long-term resistance to disease. 2o B cell treatment enhanced MOG-specific IgG1 but suppressed IgG2a antibody secretion. Cytokine profiles of splenic CD4+ T cells obtained on D21 of immunization clearly indicated that adoptively transferred 2o B cells drive Ag-specific TH2 responses. The fail of transfer of EAE with T cells from treated mice, but not control mice, supported that T cells polarized toward TH2 are responsible for the efficacy of 2o B cells. Neither regulatory T cells nor TH17 were likely to be involved in 2o B-mediated suppression of EAE. In conclusion, adoptive transfer of 2o B cells may be a promising strategy for immunotherapy of TH1-dominant autoimmune disorders. |
| Databáze: | OpenAIRE |
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