Methods for the Large-Scale Cultivation of anOxytricha(Ciliophora: Hypotrichida)1

Autor: John Michael Henry, Tommie J. Laughlin, Edwin F. Phares, Donald E. Olins, Mary V. Long
Rok vydání: 1983
Předmět:
Zdroj: The Journal of Protozoology. 30:63-64
ISSN: 0022-3921
DOI: 10.1111/j.1550-7408.1983.tb01035.x
Popis: Oxytricha strains used in biochemical studies have traditionally been grown in unaerated, unagitated culture tubes or Fernbach flasks. These cultures are limited in volume to about one liter and have a very nonuniform distribution of cells, with the majority of the cells at the very top or bottom of the medium. We have found conditions and designed experiments in which Oxytricha can be grown in 50-liter fermentation vats. The cultures grow to a uniform density of about 6000 cells/ml. 2 references, 2 figures.
Databáze: OpenAIRE