| Autor: |
Taweesak Dhammaraj, Rattazart Denchai, Surabot Suksabai, Chawannuch Mudjupa, Pawitra Pulbutr, Sakulrat Rattanakiat, Pichnatcha Nantana |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Pharmacognosy Research. 12:102 |
| ISSN: |
0974-8490 |
| DOI: |
10.4103/pr.pr_117_19 |
| Popis: |
Background: Postprandial hyperglycemia is linked with the development of diabetic complications. Inhibition against the carbohydrate-digesting enzymes, specifically pancreatic α-amylase and intestinal α-glucosidase, delays carbohydrate digestion and subsides postprandial plasma glucose (PPG) levels. Objectives: This study aimed to examine the effects of lupinifolin, purified from Derris reticulata stem, a medicinal plant traditionally used for the treatment of diabetes mellitus, on in vitro α-amylase and α-glucosidase enzyme activities and enzyme kinetics. Subjects and Methods: The identification of the isolated phytochemical was performed using nuclear magnetic resonance–mass spectrometry (NMR-MS) spectrometry.In vitro pancreatic α-amylase and α-glucosidase activities and enzyme kinetics were determined using the enzymatic colorimetric methods. Results: The purified phytochemical was identified as lupinifolin from the NMR-MS spectrometry. The isolated lupinifolin produced a significant α-amylase and α-glucosidase inhibitory actions with the median inhibitory concentrations of 3.43 ± 0.77 and 56.29 ± 11.64 μg/mL, respectively. The study of enzyme kinetics showed that lupinifolin exhibited the mode of non-competitive and mixed enzyme inhibitions against α-amylase and α-glucosidase, respectively. Conclusion: The potent α-amylase and α-glucosidase inhibitory actions of lupinifolin derived from D. reticulata stem evidently suggest its potential use as an alternative for the control of PPG. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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