Bioconversion of restaurant waste into Polyhydroxybutyrate (PHB) by recombinant E. coli through anaerobic digestion

Autor:	Majd Khalid Eshtaya, Nor ', Aini Abdul Rahman, Mohd Ali Hassan
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Environmental Engineering Chemistry Bioconversion Substrate (chemistry) law.invention Microbiology Polyhydroxybutyrate Anaerobic digestion law Yield (chemistry) Recombinant DNA Fermentation Food science Waste Management and Disposal Organic acid
Zdroj:	International Journal of Environment and Waste Management. 11:27
ISSN:	1478-9868 1478-9876
Popis:	The effect of temperature (30°C, 37°C, uncontrolled) and initial pH adjustment at pH 7 in the anaerobic digestion process was investigated to enhance the production of organic acids from restaurant waste. The highest organic acid level obtained was 39.6 g/L on the fifth day of fermentation conducted at 30°C and initial pH 7. The acids produced corresponded to 39.4% of the yield based on the initial concentration of substrate. The main organic acids produced were lactic and acetic acids. Using organic acids from fermented restaurant waste, recombinant Escherichia coli pnDTM2 gave PHB concentration, PHB content and PHB productivity of 9.2 g/L, 44% w/w and 0.54 g/L/h, respectively, in a pH stat fed-batch culture.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2d8a912730d5859761deb9467f490bd6 https://doi.org/10.1504/ijewm.2013.050521 Zobrazit plný text záznamu