Conversion of substituted naphthalenesulfonates by Pseudomonas sp. BN6

Autor: Bernd Nörtemann, A E Kuhm, Hans-Joachim Knackmuss, Andreas Stolz
Rok vydání: 1994
Předmět:
Zdroj: Archives of Microbiology. 161:320-327
ISSN: 1432-072X
0302-8933
DOI: 10.1007/bf00303587
Popis: The range of substituted naphthalenesulfonates which are metabolized by Pseudomonas sp. BN6 were investigated. Resting cells from strain BN6 oxidized 1- and 2-naphthalenesulfonate, 1-hydroxynaphthalene-2-sulfonate, 2,6-naphthalenedisulfonate and all monosulfonated naphthalene-2-sulfonates which carry one or two substitutents in the positions 4-, 5-, 6-, 7- or 8- of the naphthalene ring-system. With the exception of (substituted) 4- or 5-amino- and 4-hydroxynaphthalene-2-sulfonates these compounds were converted to the corresponding salicylates. Strain BN6 did not oxidize substituted naphthalene-1-sulfonates, 3-substituted naphthalenesulfonates and substituted naphthalenedisulfonates. Turnover of 4-amino- or 4-hydroxynaphthalene-2-sulfonates resulted in the accumulation of the corresponding naphthoquinones in the culture medium. Thus, degradation of 4-amino- and 4-hydroxynaphthalenesulfonates was restricted by the rapid autoxidation of the substituted 1,2-dihydroxynaphthalenes formed as metabolites. Catabolic activities of strain BN6 for naphthalenesulfonates were induced by salicylate, 3- or 6-hydroxysalicylate, and 3-, 4- or 5-aminosalicylate but not by 4- and 5-hydroxysalicylate. All naphthalenesulfonates that were not converted into the corresponding salicylates, were found to be inefficient as effectors. It was therefore concluded that (substituted) salicylates are the inducers of the relevant enzymes. The degradation of 2-naphthalene-sulfonate by a pure culture of strain BN6 was prevented by the toxicity of the dead-end product salicylate. Substituted salicylates were less toxic and allowed growth of strain BN6 in axenic culture with various substituted naphthalenesulfonates.
Databáze: OpenAIRE
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