The deletion of Ffh in Escherichia coli induces adverse physiological and morphological changes

Autor:	J. Y. Jiang, Xue-Ling Shen, Yong-Quan Li, H. J. Dong
Rok vydání:	2009
Předmět:	Signal recognition particle Protein subunit Mutant Biophysics Wild type Chromosomal translocation Cell Biology Biology medicine.disease_cause Biochemistry Molecular biology Cell biology Membrane protein Homologous chromosome medicine Escherichia coli
Zdroj:	Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology. 3:163-167
ISSN:	1990-7494 1990-7478
DOI:	10.1134/s1990747809020093
Popis:	The Escherichia coli Ffh protein is homologous to the SRP54 subunit of the eukaryotic signal recognition particle (SRP) that is involved in targeting and translocation of membrane proteins. The functions of Ffh in E. coli were investigated using the mutant with the Ffh deficiency. The mutant showed lower growth rate at 30°C and rapidly lost viability at the non-permissive temperature of 42°C. In addition, the amount of the total membrane proteins decreased sharply in the mutant. The mutant cells cultured at either 30 or 42°C appeared to have an elongated shape as compared to the wild type cells. Transmission electron microscopy revealed that the membrane layer of the mutant cells was thinner than that of the wild type cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2d6b1a31eee1148292407789cd86d236 https://doi.org/10.1134/s1990747809020093 Zobrazit plný text záznamu Full text from SpringerLink