Effects of flavonoids on cytochrome P-450 from rat liver microsomes: Inhibition of enzyme activities and protection against peroxidative damage

Autor: T. F. Slater, M. L. Esteve, A. Ubeda, M. J. Alcaraz, K. H. Cheeseman
Rok vydání: 1995
Předmět:
Zdroj: Phytotherapy Research. 9:416-420
ISSN: 1099-1573
0951-418X
DOI: 10.1002/ptr.2650090606
Popis: A series of flavonoids, able to inhibit non-enzymic and enzymic lipid peroxidation, were investigated as inhibitors of cytochrome P-450 mediated reactions (aminopyrine N-demethylation and aniline hydroxylation) in rat liver microsomes. The most potent compounds were chrysin, luteolin, amentoflavone and eriodictyol (aminopyrine N-demethylation) and apigenin, kaempferol and quercetin (aniline hydroxylation). Some structure-activity relationships were established for inhibition of aminopyrine N-demethylation and it was observed that the resorcinol configuration of the ring A is a determinant feature, whereas O-glycosylation abolished the activity. Most of the compounds showed lower activity on aniline hydroxylation. The presence of these flavonoids during incubation of microsomes in a medium containing NADPH did not modify the cytochrome P-450 concentration and protected against lipid peroxidation induced degradation of this cytochrome.
Databáze: OpenAIRE
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