Multilaboratory Validation of a Method To Confirm Chloramphenicol in Shrimp and Crabmeat by Liquid Chromatography-Tandem Mass Spectrometry
| Autor: | Kristl Youngs, Krystle Ferbos, Barbara K Neuhaus, Walter Hammack, Mary C Carson, Donna Kilpatrick, Jeffrey A Hurlbut, James S Stuart, David N. Heller, Cristina B Nochetto, Amy N Brown |
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| Rok vydání: | 2003 |
| Předmět: |
Pharmacology
Electrospray Chromatography Chemistry Mass spectrometry Tandem mass spectrometry Analytical Chemistry Triple quadrupole mass spectrometer Electron capture detector Liquid chromatography–mass spectrometry Environmental Chemistry media_common.cataloged_instance Gas chromatography European union Agronomy and Crop Science Food Science media_common |
| Zdroj: | Journal of AOAC INTERNATIONAL. 86:1135-1143 |
| ISSN: | 1944-7922 1060-3271 |
| Popis: | An existing method for chloramphenicol (CAP) determination in shrimp using a gas chromatograph with electron capture detector was adapted for confirmation of CAP with a liquid chromatograph interfaced to a triple quadrupole mass spectrometer. CAP residues are extracted from tissue with ethyl acetate, isolated via liquid–liquid extraction, and concentrated by evaporation. Extracts are chromatographed by using a reversed-phased column and analyzed by electrospray negative mode tandem mass spectrometry. Four product ions (m/z 152, 176, 194, and 257) of precursor m/z 321 were monitored. Moving from gas chromatography to liquid chromatography–tandem mass spectrometry improved the sensitivity of the method greatly, enabling reliable confirmation of CAP residues at 0.3 μg/kg (ppb). The method meets confirmation criteria recommended by the U.S. Food and Drug Administration and 4-point identification criteria established by the European Union. With slight modifications to accommodate different equipment, the method was validated in 3 laboratories. |
| Databáze: | OpenAIRE |
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