Lung-liquid production in vitro by lungs from fetal guinea pigs: effects of amiloride on responses to aldosterone
| Autor: | A. M. Perks, P. W. Kindler, M. Stockbrocks, D. C. Chuang, I. Vonder Muhll |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Fetus Lung Aldosterone Biology In vitro Amiloride chemistry.chemical_compound Dose–response relationship medicine.anatomical_structure Endocrinology chemistry Internal medicine medicine Gestation Animal Science and Zoology Analysis of variance Ecology Evolution Behavior and Systematics medicine.drug |
| Zdroj: | Canadian Journal of Zoology. 75:1147-1154 |
| ISSN: | 1480-3283 0008-4301 |
| DOI: | 10.1139/z97-137 |
| Popis: | Lungs from near-term fetal guinea pigs (62 ± 2 days of gestation) were supported in vitro for 3 h; lung-liquid production was monitored by a dye-dilution method based on Blue Dextran 2000. Untreated preparations produced fluid at 1.26 ± 0.14 mL∙kg−1 body mass∙h−1, with no significant change over the ensuing hours (ANOVA, regression analysis; n = 16). Experimental preparations received aldosterone at plasma concentrations reported to be present at birth. Aldosterone produced rapid, significant reductions in fluid production, and occasionally reabsorptions, which persisted beyond treatment. Reductions during treatment were as follows: 10−8 M aldosterone, 90.8 ± 4.9% (P −9 M aldosterone, 64.1 ± 16.6% (P −10 M aldosterone, 48.6 ± 11.7% (P −11 M aldosterone. Responses to 7 × 10−10 M aldosterone were abolished by 10−6 M amiloride. At the highest concentration of aldosterone (10−8 M), 10−6 M amiloride significantly reduced responses, and the changes were no longer significant by ANOVA. At both high and low aldosterone concentrations, responses with amiloride were significantly lower than those without amiloride (ANOVA, P + channels, and that the responses are remarkably rapid. |
| Databáze: | OpenAIRE |
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