| Autor: | Maria Cândida Reginato Facciotti, Gabriel Padilla, Renata L. A. Furlan, Gustavo P. Amarante-Mendes, Leandro M. Garrido, Renata Acácio Martins, Gabriela Brumatti |
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| Rok vydání: | 2002 |
| Předmět: |
Gel electrophoresis
Detection limit biology medicine.drug_class Streptomycetaceae High-throughput screening Antibiotics Bioengineering General Medicine biology.organism_classification Applied Microbiology and Biotechnology DNA Intercalation Biochemistry medicine Electrophoretic mobility shift assay Bacteria Biotechnology |
| Zdroj: | Biotechnology Letters. 24:1807-1813 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1020633909762 |
| Popis: | An improved, rapid and inexpensive gel mobility shift assay was developed for the screening of anthracycline antibiotics. The assay based on the intercalation activity of these molecules into dsDNA was used to assess the activity of partially purified antibiotics. Detection limits were of 0.1 ng ml−1 with an average run time of 2 h. The assay is potentially useful for high throughput screening in bioprospecting, for monitoring fermentation production phases and downstream purification process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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