Research Spotlight: Delivery of therapeutic RNA molecules to cancer cells by bacteria

Autor:	Hermann Lage, Johannes Fruehauf
Rok vydání:	2011
Předmět:	Effector fungi Pharmaceutical Science RNA Biology Cell biology RNA silencing chemistry.chemical_compound chemistry RNA interference DNA-directed RNA interference Transcription (biology) Cancer cell DNA
Zdroj:	Therapeutic Delivery. 2:441-449
ISSN:	2041-6008 2041-5990
DOI:	10.4155/tde.11.22
Popis:	Delivery of RNA-based therapeutics, for example RNA interference (RNAi) effectors, to target cells is one of the major obstacles for the development of RNA-based therapies. Since it has been known for a long time that bacteria can mediate tumor shrinkage, it was obvious to use nonpathogenic bacteria to produce and deliver therapeutic RNA molecules into target cells to induce RNAi. During the last years, two bacteria-based concepts were developed for this strategy, transkingdom RNAi (tkRNAi) and bacteria-mediated RNAi (bmRNAi). The first concept, tkRNAi, delivers RNAi effectors into target cells by invasive bacteria, which themselves produce therapeutic shRNAs. The bmRNAi technology utilizes invasive bacteria conveying RNAi effector-encoding DNA constructs that will act as a matrix for transcription of these sequences in the target cell by the host cell’s transcription machinery.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2d2d10533c8fbbdc0dcade2bc9435a7d https://doi.org/10.4155/tde.11.22 Zobrazit plný text záznamu