Expert consensus regarding standardization of sample preparation for clotting time assays

Autor: Osamu Kumano, Mari Emmi, Yoichi Yuki, Masaru Homma, Masahiro Ieko, Yutaka Komiyama, Masato Matsuda, Hideaki Tanaka, Kaoru Tohyama, Kazuyoshi Miyata, Yohko Kawai, Chisato Shimazu, Toshihiko Okuhara, Sumiyoshi Naito, Rika Arai, Yasuji Sekine, Hisako Katagiri, Satoshi Yamazaki, Tetsuji Yamashita, Tatsuya Yoshida
Rok vydání: 2020
Předmět:
Zdroj: International Journal of Hematology. 112:614-620
ISSN: 1865-3774
0925-5710
DOI: 10.1007/s12185-020-02983-x
Popis: Accurate clotting time assay results are vital, as the test is employed to indicate the amount of oral anticoagulant to be prescribed, while it is also used for screening the hemorrhagic and thrombotic diseases. The procedure chosen for preparation of a patient blood sample including centrifugation can contribute to significant differences in the results obtained. Thus, for the purpose of proposing a standardized method to appropriately prepare blood samples prior to assay, the Japanese Society of Laboratory Hematology organized the Working Group for Standardization of Sample Preparation for Clotting Time Assays (WG). Following reviews of previously announced guidelines and original experimental results, consensus was obtained by the WG, with the main findings as follows. (1) The recommended anticoagulant in the blood collection tube is sodium citrate solution at 0.105–0.109 M (3.13–3.2%). (2) Whole blood samples should be stored at room temperature (18–25 ˚C) within 1 h of collection from the patient. (3) For plasma preparation, centrifugation at 1500 × g should be performed for at least 15 min or at 2000 × g for at least 10 min at room temperature. (4) After the plasma sample is prepared, it should be stored at room temperature and assayed within 4 h.
Databáze: OpenAIRE
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