Biotinylated DNA probes for detecting virus Y and aucuba mosaic virus in leaves and dormant tubers of potato

Autor:	R. P. Singh, Mounir G. AbouHaidar, D. Leclerc, M. Eweida
Rok vydání:	1992
Předmět:	Mosaic virus Potato aucuba mosaic virus fungi Potyvirus food and beverages RNA virus Biology biology.organism_classification Potexvirus Virology Molecular biology Virus Potato virus Y Plant virus Agronomy and Crop Science Food Science
Zdroj:	Potato Research. 35:173-182
ISSN:	1871-4528 0014-3065
DOI:	10.1007/bf02357611
Popis:	Detection of potato virus Y (PVY) in dormant potato tubers using the enzyme-linked immunosorbent assay (ELISA) has been reported not to be accurate and reliable and it requires breaking of the dormancy of tubers prior to testing. We describe a simple, practical and highly sensitive hybridization method based on the use of biotinylated DNA probes for detecting PVY and potato aucuba mosaic virus (PAMV) in dilutions made of crude extracts of infected potato leaves and dormant tubers. As little as 50 fg of RNA can be detected by this method, and the probes are highly specific for their targets, even in crude plant extracts. The presence of one virus did not interfere with the detection of the other.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2d22fda9724d460bd9e986319c94eaf4 https://doi.org/10.1007/bf02357611 Zobrazit plný text záznamu Full text from SpringerLink