Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron v1
Autor: | Lori Zeltser |
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Rok vydání: | 2022 |
DOI: | 10.17504/protocols.io.261generdg47/v1 |
Popis: | A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat. Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB) Step 2: Ganglia dissociation and single-cell sorting Step 3: Single-cell sequencing and analysis |
Databáze: | OpenAIRE |
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