Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron v1

Autor: Lori Zeltser
Rok vydání: 2022
DOI: 10.17504/protocols.io.261generdg47/v1
Popis: A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat. Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB) Step 2: Ganglia dissociation and single-cell sorting Step 3: Single-cell sequencing and analysis
Databáze: OpenAIRE