Controlled protein adsorption on PMOXA/PAA based coatings by thermally induced immobilization
| Autor: | Fatima Mumtaz, Chao Pan, Yanmei Wang, Haikun Zhu, Chaoshi Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Spin coating
Glycidyl methacrylate Materials science General Physics and Astronomy Chain transfer 02 engineering and technology Surfaces and Interfaces General Chemistry biochemical phenomena metabolism and nutrition 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Methacrylate 01 natural sciences 0104 chemical sciences Surfaces Coatings and Films chemistry.chemical_compound chemistry Polymerization Chemical engineering Copolymer 0210 nano-technology Acrylic acid Protein adsorption |
| Zdroj: | Applied Surface Science. 439:148-159 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2018.01.010 |
| Popis: | In this work, poly(2-methyl-2-oxazoline-random-glycidyl methacrylate) (PMOXA-r-GMA) and poly(acrylic acid)-block-poly(glycidyl methacrylate) (PAA-b-PGMA) copolymers were synthesized via cationic ring-opening polymerization (CROP) of 2-methyl-2-oxazoline (MOXA) and reversible addition-fragmentation chain transfer (RAFT) polymerization of acrylic acid (AA) followed by their random and block copolymerization with glycidyl methacrylate (GMA), respectively, and then characterized carefully. PMOXA/PAA based coatings were then prepared by simply spin coating the mixture of PMOXA-r-GMA and PAA-b-PGMA copolymer solutions onto silicon/glass substrates followed by annealing at 110 °C. The coatings were rigorously characterized by using X-ray photoelectron spectroscopy (XPS), the static water contact angle (WCA) test, ellipsometry and atomic force microscopy (AFM). The results demonstrated that the coating based mixed PMOXA/PAA brushes with desired surface composition could be attained by simply maintaining their percentage in the mixture of PMOXA-r-GMA and PAA-b-PGMA copolymer solutions. Finally, the switchable behavior of PMOXA/PAA based coatings toward bovine serum albumin (BSA) adsorption was investigated by fluorescein isothiocyanate-labelled BSA (FITC-BSA) assay and quartz crystal microbalance with dissipation monitoring (QCM-D), which indicated that the coating based mixed PMOXA/PAA brushes could control BSA adsorption/desorption from very low to high amount (>90% desorption) through adjusting the composition of PMOXA-r-GMA and PAA-b-PGMA solution used in preparing PMOXA/PAA based coatings upon pH and ionic strength change. Furthermore, PMOXA/PAA based coatings displayed efficient repeatability of reversible BSA adsorption/desorption cycles. |
| Databáze: | OpenAIRE |
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