Sensibilización y alergia a látex en residentes quirúrgicos del Hospital General de México
| Autor: | Andrea Aida Velasco-Medina, Javier Hernández-Romero, Mariana Esther Arroyo-Cruz, Rosa Isela Campos-Gutiérrez, Guillermo Velázquez-Sámano, Rodrigo Collado-Chagoya, Ana del Carmen García-González, Alejandro Eliosa Alvarado-Gumaro |
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| Rok vydání: | 2018 |
| Předmět: |
House dust mite
Allergy biology medicine.diagnostic_test business.industry biology.organism_classification medicine.disease Immunoglobulin E Epitope respiratory tract diseases 03 medical and health sciences 0302 clinical medicine Immune system Bronchoalveolar lavage 030228 respiratory system Immunology Blocking antibody biology.protein Immunology and Allergy Medicine 030212 general & internal medicine Antibody business |
| Zdroj: | Revista Alergia México. 65:128-139 |
| ISSN: | 2448-9190 0002-5151 |
| Popis: | Background: The mite Blomia tropicalis (Blo t) is an important source of allergens and a major risk factor for allergic rhinitis and asthma. A hybrid protein that includes epitopes of allergens Blo t 1, Blo t 5, Blo t 10, Blo t 12 and Blo t 13 (BTx5) and shows low IgE reactivity in sera from house dust mite allergic subjects was reported previously. This study evaluated the BTx5 immune response in BALB/c mice. Methods: Mice were immunized intraperitoneally with BTx5 mixed with aluminum hydroxide and intranasal challenge with BTx5. Additionally, a group of mice was similarly treated with Blomia tropicalis and another group with PBS. Serum levels of specific IgE, IgG1, IgG2a and cytokines in bronchoalveolar lavage fluid (BALF) were determined by ELISA. Airway inflammatory response was evaluated by methacholine challenge and the cellular infiltrate in lung tissue. The blocking effect of the IgG raised in mice immunized with BTx5 on IgE binding to Blo t extract was analyzed by ELISA inhibition. Results: Immunization with BTx5 induced specific IgG2a to Blomia tropicalis extract; the antibody levels were significantly higher than IgE (IgE/IgG2 = 0.22). Mice treated with BTx5 versus Blomia tropicalis extract showed significantly lower levels of IL-5 (p = 0.05) and IL-13 in BALF (p = 0.01), airway hyper-reactivity (p = 0.01), cellular infiltration (p = 0.008) and mucus production in lung tissue (p = 0.045). The BTx5 induced IgG antibodies inhibited 63 % of the IgE reactivity to Blomia tropicalis extract in human sera from allergic patients sensitized to Blomia tropicalis. Conclusions: BTx5 fosters a Th1 biased immune response and lower airway inflammation. The induction of IgG blocking antibodies suggests a potential anti-allergic effect. |
| Databáze: | OpenAIRE |
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