Influence of ovariectomy on the cytokine release in acute lung injury induced by LPS in mice (P5052)
| Autor: | Joao Gimenes-Junior, Luana Vitoretti, Beatriz Accetturi, Adriana Lino-dos-Santos-Franco, Ana Ligeiro-de-Oliveira, Jean Peron, Thiago Aloia, Joao Palermo-Neto, Ricardo Oliveira-Filho, Wothan Tavares-de-Lima |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 190:180.9-180.9 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.190.supp.180.9 |
| Popis: | Acute respiratory distress syndrome is a multifactorial disease characterized by acute lung injury, hypoxemia and polymorphonuclear cells infiltration. Clinical and experimental evidences indicate that female sex hormones may greatly influence lung inflammation. Previous data from our group revealed that ovariectomy (OVx) blunted LPS-induced lung inflammation in mice after 24 h. Here, we expanded the study and assessed the influence of OVx on the release of cytokines by lung cells after LPS. 7 day-OVx female C57BL/6 mice were subjected to intranasal instillation of LPS or saline. Non-manipulated animals were subjected to the same procedures and served as controls (Sham-OVx). 24 h thereafter, animals were euthanized and lungs removed. The levels of IL-1β, IL-17, IFN-γ and IL-10 were measured. Ovaries removal per se did not alter the cytokines levels in the lung compared with the control group. Moreover, intranasal instillation of LPS increased the cytokines released in the lungs from both OVx and Sham-OVx groups compared to controls. On the other hand, LPS challenged animals had more IL-1β, IL-17 and IFN-γ within the OVx group. This was associated with significantly higher levels of IL-10 in the same group. Lack of female sex hormones led to an increased release of pro-inflammatory cytokines by the lungs, demonstrating its role in modulating the development of acute lung injury induced by LPS in mice. |
| Databáze: | OpenAIRE |
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