| Autor: |
Darshan S. Brar, S. Rambold, O.L. Gamborg, Friedrich Constabel |
| Rok vydání: |
1979 |
| Předmět: |
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| Zdroj: |
Zeitschrift für Pflanzenphysiologie. 95:377-388 |
| ISSN: |
0044-328X |
| DOI: |
10.1016/s0044-328x(79)80208-1 |
| Popis: |
Summary A fast growing cell suspension culture of sorghum ( Sorghum bicolor L. Moench ) has been established. Out of 15 genetic strains, cv. GPR-168 responded most favorably to conditions for callus formation. The callus was friable and upon transfer to liquid medium produced cell suspension cultures. During 96 h of subculturing the cells showed mitotic indices of 1.0 to 6.5 %. Plant regeneration from callus derived from seedlings and immature embryos was achieved in two sorghum strains. Cytology of callus, cell suspension cultures, and regenerated plants indicated a strong prevalence of polyploidization. The study also includes observations on a fast growing cell culture of corn. The culture had a doubling time of 48 h and during a 72 h period showed mitotic indices from 3.6 to 10.9 %. Protoplasts were readily isolated from the cell culture, but spontaneous fusion occurred extensively. On average 52 % of the isolated protoplasts were multinucleated. Attempts to eliminate the phenomenon were not successful. During subsequent culture some of the protoplasts regenerated cell walls and limited division occurred. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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