Accuracy of complement activation product levels to detect infected pleural effusion in rats
| Autor: | Eliane Roesch, Jane Maria Ulbrich Kulczynski, Sergio Luis Amantea, Samanta Sarmento da Silva, José Carlos Soares de Fraga, Patrícia Miorelli, Guilherme Eckert Peterson, Paulo Roberto Stefani Sanches |
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| Rok vydání: | 2017 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Pathology Pleural effusion business.industry Pleural empyema medicine.medical_treatment Area under the curve Thoracentesis medicine.disease medicine.disease_cause Gastroenterology Empyema Complement system 03 medical and health sciences 0302 clinical medicine 030228 respiratory system Internal medicine Pediatrics Perinatology and Child Health Streptococcus pneumoniae medicine 030212 general & internal medicine Complication business |
| Zdroj: | Pediatric Pulmonology. 52:757-762 |
| ISSN: | 8755-6863 |
| Popis: | Summary Background: Pleural empyema is a well-known complication of pneumonia. If treatment is delayed, empyema may increase morbidity and mortality in affected patients. Therefore, the identification of empyema biomarkers in parapneumonic pleural effusion is desirable. Previous research has suggested complement activation products as candidate empyema markers. Objective: To compare the levels of complement activation products C3a, C5a, and C5b9 in pleural effusion induced by Staphylococcus aureus (SA), Streptococcus pneumoniae (SP), or turpentine (control). Methods: Thirty-nine male Wistar rats (mean weight 414 g; 290–546 g) were allocated as follows: 17 animals in the SA group, 12 in the SP group, and 10 in the control group. Bacteria or turpentine were injected into the pleural space. After 12 hr, intrapleural fluid was collected using ultrasound-guided thoracentesis. Levels of complement activation products were determined using ELISA kits. Results: Two SA and one SP animals died before 12 hr. Mean levels were as follows: C3a: 1066.82 µg/ml (937.29–1196.35 µg/ml) in SA, 1188.28 µg/ml (1095.65–1280.92 µg/ml) in SP, and 679.13 µg/ml (601.29–756.98 µg/ml) in controls (P |
| Databáze: | OpenAIRE |
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