Effects of mRNA, Protein Expression and Activity for Myocardial SOD2 by a Single Bout of or Long-Term Strenuous Endurance Exercise in Rats
| Autor: | Simao Xu, Weichun Liu, Minhua Li |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Messenger RNA
medicine.medical_specialty business.industry musculoskeletal neural and ocular physiology Mrna expression SOD2 General Medicine General Chemistry SOD2 protein Protein expression Endocrinology Endurance training Internal medicine cardiovascular system medicine skin and connective tissue diseases business human activities |
| Zdroj: | Chinese Medicine. :251-258 |
| ISSN: | 2151-1926 2151-1918 |
| DOI: | 10.4236/cm.2014.54030 |
| Popis: | Objective: To explore the effects of myocardial SOD2 by strenuous endurance exercise. Methods: 27 grown male SD rats were randomly divided into control group (C), a single bout of strenuous endurance exercise group (E1) and seventh-week strenuous endurance exercise group (E2). Real-time PCR was used to observe the changes of mRNA expression for myocardial SOD2. Western bolt was used to observe the changes of SOD2 protein expression. In addition, SOD2, T-SOD and SOD1 activity changes were observed. Results: Myocardial SOD2 expression level at mRNA and protein of Group E1, E2 was significantly higher than that in group C, and SOD2 and T-SOD activity in group E2 were significantly higher than those in group C. Those changes were more obvious in group E2. Conclusions: Strenuous endurance exercise can improve level of myocardial SOD2 expression at mRNA and protein, and enhance the activity for SOD2, thus increasing the activity for T-SOD. Effect of long-term strenuous endurance exercise was better than a single bout of one. |
| Databáze: | OpenAIRE |
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