Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts
| Autor: | Monika Pávková Goldbergová, Petr Babula, Jana Veverková, Jan Balvan, Josef Mašek, Pavel Kulich, Michal Štefančík, Tomas Vicar, Lucie Válková |
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| Rok vydání: | 2020 |
| Předmět: |
biology
Chemistry Health Toxicology and Mutagenesis Nanoparticle Osteoblast General Medicine Adhesion 010501 environmental sciences 01 natural sciences Pollution medicine.anatomical_structure Tubulin Cell culture Gene expression Biophysics medicine biology.protein Environmental Chemistry Cytoskeleton Actin 0105 earth and related environmental sciences |
| Zdroj: | Environmental Science and Pollution Research. 28:6018-6029 |
| ISSN: | 1614-7499 0944-1344 |
| Popis: | Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO2 and Ni are released. This study is focused on the impact of TiO2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO2 nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO2 nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells. Graphical abstract. |
| Databáze: | OpenAIRE |
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