Evaluation of chlorophyll fluorescence, in vivo spectrophotometric pigment absorption and ion leakage as biomarkers of UV-B exposure in marine macroalgae
| Autor: | Michael H. Depledge, B. Cordi, L. F. Salter, D. N. Price, Maria E. Donkin |
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| Rok vydání: | 1997 |
| Předmět: |
Ecology
biology Phycoerythrobilin Photosynthetic pigment Chlorophyta Aquatic Science biology.organism_classification chemistry.chemical_compound chemistry Palmaria palmata Chlorophyll Environmental chemistry Phycocyanin Botany biology.protein Phycoerythrin Chlorophyll fluorescence Ecology Evolution Behavior and Systematics |
| Zdroj: | Marine Biology. 130:41-49 |
| ISSN: | 1432-1793 0025-3162 |
| Popis: | The photosynthetic fluorescence ratio Fv:Fm, in vivo absorption spectra and ion leakage were evaluated as biomarkers of ambient and elevated UV-B (280 to 320 nm) exposure of the intertidal alga Enteromorpha intestinalis (Chlorophyta) and the sublittoral alga Palmaria palmata (Rhodophyta). Measurements of thallus growth were also used to assess adverse biological effects. Ambient and elevated UV-B significantly inhibited photosynthesis in both species. It was shown that the Fv:Fm ratio is a sensitive, non-specific general biomarker of UV-B exposure in both species. Moreover, the in vivo absorption of what was tentatively identified as chlorophylls a and b as well as phycoerythrin and/or carotenoids, phycoerythrobilin and phycocyanin decreased in a dose-response dependent manner and was associated with a decrease in growth rate in P. palmata. The intertidal alga E. intestinalis showed a greater degree of tolerance to UV-B exposure. These results indicate that changes in the Fv:Fm ratio together with reductions in in vivo pigment absorption could provide an early quantitative warning of the detrimental effects of UV-B in marine macroalgae. |
| Databáze: | OpenAIRE |
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