Integrated Proteomics and Transcriptomics Analyses Reveals the Possible Antifungal Mechanism of an Indoloquinoline Alkaloid Neocryptolepine against Rhizoctonia solani
| Autor: | Jiyu Zhang, Zhi-Jun Zhang, Jia-Kai Zhu, Wu Tianlin, Li-Xia Dai, Xiaolou Miao, Ying-Qian Liu, Ying-Hui He, Yin-Fang Yan, Du Shasha, Xiao-Fei Shang, Cheng-Jie Yang |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
biology Cytochrome Chemistry Alkaloid 010401 analytical chemistry food and beverages General Chemistry Oxidative phosphorylation biology.organism_classification 01 natural sciences 0104 chemical sciences Rhizoctonia solani Mitochondrial respiratory chain Biochemistry Coenzyme Q – cytochrome c reductase biology.protein General Agricultural and Biological Sciences Mode of action Mycelium 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:6455-6464 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Rhizoctonia solani causes serious plant diseases. Neocryptolepine presented the significant antifungal activity against R. solani, however the mode of action is unclear. In this paper, we investigated the potential mode of action of neocryptolepine against R. solani integrated the proteomics and transcriptomics. Results showed that after treatment with neocryptolepine, 1012 differentially expressed proteins and 10 920 differentially expressed genes of R. solani were found, most of them were enriched in mitochondrial respiratory chain. It affected oxidative phosphorylation led to the enrichment of ROS and the decrease of MMP, and inhibited complex III activity with the inhibition rate of 63.51% at 10 μg/mL. The mitochondrial structural and function were damaged. Cytochrome b-c1 complex subunit Rieske (UQCRFS1) with the high binding score to neocryptolepine was found as a potential target. In addition, it inhibited the sclerotia formation and presented antifungal efficacy by decreasing the diameter of a wound in potato in a concentration-dependent manner. Above results indicated that neocryptolepine inhibited the complex III activity by binding UQCRFS1 and blocked the ion transfer to cause the death of R. solani mycelia. This study laid the foundation for the future development of neocryptolepine as an alternative biofungicide. |
| Databáze: | OpenAIRE |
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