| Autor: |
Heather A. H. Haemig, Gary M. Dunny |
| Rok vydání: |
2014 |
| Předmět: |
|
| DOI: |
10.1128/9781555815578.ch3 |
| Popis: |
One of the best-studied forms of microbial behavior controlled by intercellular signaling is pheromone-inducible conjugation in Enterococcus faecalis. This chapter emphasizes significant results of research on the pheromone systems during the past several years, particularly the pCF10 system. The chapter focuses on new insights into the control of pheromone activity in donor and recipient cells, on the molecular mechanism of the pheromone response, and on the evolution of pCF10 plasmids. The majority of research on pCF10 plasmids has focused on pAD1 and pCF10. The genes encoded on the approximately 70-kb pCF10 plasmid are involved in pheromone-inducible conjugation. Specific outcomes of pheromone induction are discussed in the chapter. The authors describe the synthesis of cCF10 to exemplify a process that occurs in a similar fashion for several pheromones. The genetic screens and biochemical evidence demonstrating two functions for PrgX resulted in the development of a model of how PrgX negatively regulates from the prgQ promoter as well as positively regulates its own expression by a single mechanism. The X-ray crystal structure of PrgX is mainly helical with 17 alpha-helices. The authors propose that the pCF10 system described in the chapter has been appropriated by the plasmid for the purpose of increasing the sensitivity and adaptability to multiple situations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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