Hybrid graphene oxide as carrier of doxorubicin: cytotoxicity and preliminary in vivo assays against bladder cancer
| Autor: | Luiz Alfredo Braun Ferreira, Marcela Durán, Marcelo Bispo de Jesus, Joel Gonçalves de Souza, Petra Karla Bockelmann, Juliana S. Bernardes, Wagner José Fávaro, Nelson Durán |
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| Rok vydání: | 2020 |
| Předmět: |
technology
industry and agriculture RNA macromolecular substances 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Industrial and Manufacturing Engineering 0104 chemical sciences Nanomaterials Vascular endothelial growth factor chemistry.chemical_compound chemistry In vivo Attenuated total reflection Zeta potential Biophysics medicine General Materials Science Doxorubicin Electrical and Electronic Engineering 0210 nano-technology Cytotoxicity medicine.drug |
| Zdroj: | Advances in Natural Sciences: Nanoscience and Nanotechnology. 11:025016 |
| ISSN: | 2043-6262 |
| DOI: | 10.1088/2043-6254/ab9194 |
| Popis: | Two types of graphene oxide (GO) hybrids were synthesised for the administration of doxorubicin (DOX, named GO-CO-DOX) and interfering RNA - siRNA (named GO-PEG-PEI/siRNA). These nanomaterials were used for intervention on vascular endothelial growth factor (VEGF) that represents an important strategy to block the formation of new vessels to inhibit cancer progression. For the delivery of DOX, it was incorporated into GO, while for the delivery of siRNA, GO was covalently bonded with the cationic polyethyleneimine (PEI) and then complexed with siRNA. The nanostructures were characterised by attenuated total reflection ATR-FTIR, zeta potential, x-ray photoelectron spectroscopy (XPS), x-ray diffraction (XRD), and transmission electron microscopy (TEM). The cytotoxicity studies with GO-PEG-PEI and GO-PEG-PEI/siRNA systems showed that both formulations have IC50 values of around 100 μg ml−1. The systems were administered in vivo to investigate their antitumor effects against non-muscle-invasive bladder cancer (NMIBC) and showed to be promising for the treatment of NMIBC. |
| Databáze: | OpenAIRE |
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