Preparation of Peptide Thioesters using Fmoc-Solid-Phase Peptide Synthesis and its Application to the Construction of a Template-Assembled Synthetic Protein (TASP)

Autor:	Mineo Niwa, Shiroh Futaki, Hironobu Hojo, Jun Maruyama, Koji Sogawa, Tomoko Asahara
Rok vydání:	1997
Předmět:	chemistry.chemical_classification Synthetic protein Aqueous solution Chemistry Organic Chemistry Peptide Thioester Biochemistry Combinatorial chemistry chemistry.chemical_compound Transmembrane domain Phase (matter) Drug Discovery Peptide synthesis lipids (amino acids peptides and proteins)
Zdroj:	Tetrahedron Letters. 38:6237-6240
ISSN:	0040-4039
DOI:	10.1016/s0040-4039(97)01434-2
Popis:	Preparation of peptide thioesters was conducted through peptide chain construction with Fmoc-solid-phase peptide synthesis on a 2-chlorotrityl resin followed by coupling with HS-(CH2)2-COOEt and deprotection with 95% aqueous CF3COOH. The peptide thioester corresponding to a transmembrane segment of the calcium channel (S4 in repeat IV) thus obtained was introduced onto a peptide template to give an artificial four-helix-bundle protein. © 1997 Elsevier Science Ltd.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2c5d1c137475f19589011c670f033c5b https://doi.org/10.1016/s0040-4039(97)01434-2 Zobrazit plný text záznamu Full Text from ScienceDirect