The pleiotropic nature of rib80, hit1, and red6 mutations affecting riboflavin biosynthesis in the yeast Pichia guilliermondii

Autor:	Dariya V. Fedorovych, Andriy A. Sibirny, Yu. R. Boretsky, T. M. Prokopiv, Lyubov R. Fayura
Rok vydání:	2007
Předmět:	chemistry.chemical_classification Pichia guilliermondii Mutation Riboflavin Iron deficiency Biology medicine.disease medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Aconitase Yeast Enzyme Biochemistry chemistry medicine Overproduction
Zdroj:	Microbiology. 76:55-59
ISSN:	1608-3237 0026-2617
DOI:	10.1134/s0026261707010080
Popis:	The yeast Pichia guilliermondii is capable of riboflavin overproduction under iron deficiency. The rib80, hit1, and red6 mutants of this species, which exhibit impaired riboflavin regulation, are also distinguished by increased iron concentrations in the cells and mitochondria, morphological changes in the mitochondria, as well as decreased growth rates (except for red6) and respiratory activity. With sufficient iron supply, the rib80 and red6 mutations cause a 1.5–1.8-fold decrease in the activity of such Fe-S cluster proteins as aconitase and flavocytochrome b 2, whereas the hit1 mutation causes a six-fold decrease. Under iron deficiency, the activity of these enzymes was equally low in all of the studied strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2c3cc839d8e459f79109a7a65aeac7b0 https://doi.org/10.1134/s0026261707010080 Zobrazit plný text záznamu Full text from SpringerLink